T. Osada et al., The distribution of sugar chains on the vomeronasal epithelium observed with an atomic force microscope, CHEM SENSE, 24(1), 1999, pp. 1-6
The distribution of sugar chains on tissue sections of the rat vomeronasal
epithelium, and the adhesive force between the sugar and its specific lecti
n were examined with an atomic force microscope (AFM). AFM tips were modifi
ed with a lectin, Vicia villosa agglutinin, which recognizes terminal N-ace
tyl-D-glactosamine (GalNAc). When a modified tip scanned the luminal surfac
e of the sensory epithelium, adhesive interactions between the tip and the
sample surface were observed. The final rupture force was calculated to be
similar to 50 pN based on the spring constant of the AFM cantilever. Distri
bution patterns of sugar chains obtained from the force mapping image were
very similar to those observed using fluorescence-labeled lectin staining.
AFM also revealed distribution patterns of sugar chains at a higher resolut
ion than those obtained with fluorescence microscopy. Most of the adhesive
interactions disappeared when the scanning solution contained 1 mM GalNAc.
The adhesive interactions were restored by removing the sugar from the solu
tion. Findings suggest that the adhesion force observed are related to the
binding force between the lectin and the sugars distributed across the vome
ronasal epithelium.