Objective
The aim of this study was to determine the HLA antigens in Mexican Mestizo
patients with mixed connective tissue disease (MCTD).
Methods
We studied 30 patients with MCTD and 99 healthy controls. HLA-A, -B, and DQ
antigens were typed by microlymphocytotoxicity assays. DRB1, DQA1 and DQB1
alleles were oligotyped.
Results
HLA-A2 and HLA-B35 were the most frequent MHC class I alleles in MCTD patie
nts, although they were not statistically more frequent than in the control
s. According to serological tests, the most frequent DQ allele in the patie
nts was DSI, which was statistically increased when compared with controls
(p = 0.0051). By oligotyping, the DR1 allele and the DQB1* 0501 specificiti
es were significantly increased in the patients vs. controls (p = 0.032 and
0.027, respectively).
Conclusion
The elevated levels of DQ1 found in Mexican MCTD patients, although weak, m
ay indicate a particular genetic susceptibility, since there are previous r
eports of associations of other alleles (such as DR4) with MCTD in other po
pulations. The increase in DQB1* 0501 may account for the increase in DQ1 D
QB1*0501 has also been reported in black patients with anti-RNP autoantibod
ies, compared with black patients without anti-RNP or anti-Sm.