Y. Kizawa et al., Antigen-induced elevation of immunoreactive endothelin-1 (ET-1) levels in ovalbumin-sensitized guinea pig airway tissue, COMP BIOC C, 122(2), 1999, pp. 239-243
Citations number
30
Categorie Soggetti
Pharmacology & Toxicology
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY
Changes in the immunoreactive ET-I levels during the anaphylactic reaction
of airway tissue from ovalbumin-sensitized guinea pigs were investigated. E
T-l-immunoreactivity (ET-IR) was detected in the epithelial and smooth musc
le layers of tracheal sections from normal guinea pigs and it was enhanced
slightly by phosphoramidon (I mu M) treatment. The ET-IR level of the epith
elial layer of ovalbumin-treated tissue from actively sensitized animals wa
s slightly higher than that from normal animals, but it was enhanced marked
ly by phosphoramidon (1 mu M) treatment. Furthermore, the mean ET-IR level
of homogenates of antigen-treated tracheal tissues from sensitized guinea p
igs (22.8 +/- 1.55 fmol mg(-1) protein, n = 5) was significantly higher tha
n the corresponding normal level (12.3 +/- 1.21 fmol mg(-1) protein, n = 5)
. These results suggest that increased epithelial airway ET-1 levels contri
bute to the anaphylactic reaction of guinea pig airways. (C) 1999 Elsevier
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