Inhibition of nucleoside diphosphate kinase in rat liver mitochondria by added 3 '-azido-3 '-deoxythymidine

The effect of 3'-azido-3'-deoxythymidine on nucleoside diphosphate kinase o f isolated rat liver mitochondria has been studied. This is done by monitor ing the increase in the rate of oxygen uptake by nucleoside diphosphate (TD P, UDP, CDP or GDP) addition to mitochondria in state 4, It is shown that 3 '-azido-3'-deoxythymidine inhibits the mitochondrial nucleoside diphosphate kinase in a competitive manner, with a K-1 value of about 10 mu M as measu red for each tested nucleoside diphosphate, It is also shown that high conc entrations of GDP prevent 3'-azido-3'-deoxythymidine inhibition of the nucl eoside diphosphate kinase. (C) 1999 Federation of European Biochemical Soci eties.