Human nerve growth factor beta (hNGF-beta): mammary gland specific expression and production in transgenic rabbits

Authors
Coulibaly, S Besenfelder, U Fleischmann, M Zinovieva, N Grossmann, A Wozny, M Bartke, I Togel, M Muller, M Brem, G
Citation
S. Coulibaly et al., Human nerve growth factor beta (hNGF-beta): mammary gland specific expression and production in transgenic rabbits, FEBS LETTER, 444(1), 1999, pp. 111-116
Citations number
39
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
444
Issue
1
Year of publication
1999
Pages
111 - 116
Database
ISI
SICI code
0014-5793(19990205)444:1<111:HNGFB(>2.0.ZU;2-2
Abstract
Transgenic rabbits carrying gene constructs encoding human nerve growth fac tor beta (hNGF-beta) cDNA were generated. Expression of hNGF-beta mRNA was restricted to the mammary gland of lactating rabbits. Western Blot analysis revealed a polypeptide of 13.2 kDa in the milk of transgenic animals, hNGF -beta was purified from the milk by a two-step chromatographic procedure, E lectrospray mass spectroscopy analysis of purified hNGF-beta depicted a mol ecular weight of 13 261 Da per subunit. The biological activity of the hNGF -beta was tested using PC12W2 cells and cultures of dorsal root ganglion ne urons from chicken embryos. Crude defatted milk from transgenic animals and purified hNGF-beta demonstrated full biological activity when compared to commercial recombinant hNGF-beta. (C) 1999 Federation of European Biochemic al Societies.