Induction of humoral and cellular immune responses in mice by a recombinant fowlpox virus expressing the E2 protein of bovine viral diarrhea virus

Authors
Elahi, SM Bergeron, J Nagy, E Talbot, BG Harpin, S Shen, SH Elazhary, Y
Citation
Sm. Elahi et al., Induction of humoral and cellular immune responses in mice by a recombinant fowlpox virus expressing the E2 protein of bovine viral diarrhea virus, FEMS MICROB, 171(2), 1999, pp. 107-114
Citations number
27
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
03781097 → ACNP
Volume
171
Issue
2
Year of publication
1999
Pages
107 - 114
Database
ISI
SICI code
0378-1097(19990215)171:2<107:IOHACI>2.0.ZU;2-6
Abstract
A recombinant fowlpox virus (rFPV/E2) expressing the E2 protein of bovine v iral diarrhea virus (BVDV) was constructed and characterized. Mice were imm unized with recombinant virus and both humoral and cellular immune response s were studied. rFPV/E2 induced BVDV-specific antibodies which were detecte d by ELISA. In addition, mouse sera were shown to neutralize BVDV. A cytoki ne ELISA assay revealed that mice vaccinated with rFPV/E2 induced 7-fold mo re interferon-gamma than parental fowlpox virus. (C) 1999 Published by Else vier Science B.V. All rights reserved.