Y. Wang et al., Multiple forms of glucagon and somatostatin isolated from the intestine ofthe southern-hemisphere lamprey Geotria australis, GEN C ENDOC, 113(2), 1999, pp. 274-282
Current views on Agnathan phylogeny favor the hypothesis that the genera of
holarctic lampreys belong to a single family (Peuomyzontidae) and form an
interrelated progression in which Petromyzon is near to Ichthomyzon at the
base of the phylogenetic tree and Lampetra is the most derived. A stock sim
ilar to that of contemporary Ichthomyzon is considered to have given rise t
o the southern hemisphere lamprey Geotria australis, the sole member of the
Geotriidae. In the present study, two molecular forms of glucagon were iso
lated from an extract of G. australis intestine that differed in structure
by six amino acid residues. One form shows two amino acid substitutions (Le
u(14) --> Met and Ala(29) --> Ser) compared with the single molecular form
of glucagon isolated from the sea lamprey Petromyzon marinus and the second
form shows three substitutions (Asp(15) --> Glu, Ser(16) --> Ala, IIe(24)
--> Thr) compared with the single glucagon isolated from the river lamprey
Lampetra fluviatilis. As Petromyzon and Lampetra glucagons differ by six am
ino acid residues, the data suggest that a duplication of the glucagon gene
occurred prior to or early in lamprey evolution. Although both genes are s
trongly expressed in G. australis, the expression of one gene predominates
in P. marinus while that of the other gene predominates in L. fluviatilis.
Previous work has shown that, in the islet organ of G. australis, preprosom
atostatin is processed almost exclusively to somatostatin-33. However, the
present study demonstrates that somatostatin-14 is the major molecular form
in G. australis intestine with somatostatin-33 present only as a minor com
ponent. This result demonstrates a tissue-dependent pathway of posttranslat
ional. processing of preprosomatostatin in the Geotria enteropancreatic sys
tem. (C) 1999 Academic Press.