Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling

Authors
Masso, F Varela, E Paez, A Zenteno, E Montano, LF
Citation
F. Masso et al., Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling, GLYCOCON J, 15(9), 1998, pp. 843-846
Citations number
21
Categorie Soggetti
Biochemistry & Biophysics
Journal title
GLYCOCONJUGATE JOURNAL
ISSN journal
02820080 → ACNP
Volume
15
Issue
9
Year of publication
1998
Pages
843 - 846
Database
ISI
SICI code
0282-0080(199809)15:9<843:IOMGFM>2.0.ZU;2-M
Abstract
To identify Mycobacterium bovis glycoproteins, carbohydrates present in a d elipidized M. bovis culture filtrate protein extract were biotin-hydrazide labeled. 11 carbohydrate-containing protein with a molecular weight of 15-, 19-, 25-, 32-, 35-, 39-, 42-, 48-, 52-, 58-, and 62-kDa were detected. The 52- and 32-kDa protein were deglycosylated by endoglycosidase-F.