Autocrine B-cell stimulation by interleukin-2 during a cognate interactionwith T-cells

Interleukin-2 (IL-2) secretion as well as expression of IL-2 receptor has b een demonstrated for B-cells in response to several activating stimuli. How ever, the exact role of B-cell-derived IL-2 in the T-cell-dependent antibod y response remains to be determined. Here, we have examined the autocrine r egulatory roles of IL-2 secreted from B-cells. Splenic resting B-cells were stimulated with a fixed pre-activated Th1 clone, G1.19, in the presence of a single amino acid-substituted peptide (pD129A; Ala-129 substituted for A sp-129), an analog of the original ligand (p119-133, derived from bovine be ta-lactoglobulin) recognized by G1.19 cells. pD129A allowed a cognate inter action between B-cells and fixed pre-activated G1.19 T-cells, but pD129A ha d no agonistic activity against G1.19 T-cells. Thus, the level of expressio n of B-cell-activating molecules on T-cells remained unchanged after stimul ation with pD129A. Regardless of the lack of ability to induce IL-2 secreti on in the case of T-cells, pD129A significantly enhanced antibody secretion from B-cells, and this was partially blocked by anti-IL-2 antibody. Furthe rmore, IL-2 secretion from B-cells was modestly upregulated in response to added pD129A. Taken together, these data suggest that helper signals from i nteracting cognate T-cells induce IL-2 secretion by B-cells, which can enha nce antibody secretion in an autocrine manner. (C) 1999 Elsevier Science B. V. All rights reserved.