A comparative utilization of exogenous and endogenous substrates by the spermatozoa of buffaloes from testis and excurrent duct after incubation at 37 degrees C for two hours

The endogenous respiration of the washed buffalo testicular epididymal and vas spermatozoa was studied by estimating their lipid, phospholipid and cho lesterol contents. The utilization of exogeneous substrates was recorded to be about 50% by the testicular spermatozoa which increased through vas-def erens up to 100%. The endogenous substrates could be utilized for energy pr oduction in the absence of exogenous substrates. The testicular spermatozoa posses the capability of metabolizing the substrates even at the time of s permatogenesis, which went on increasing with the maturation of the spermat ozoa from testis through epididymis and vas-deferens.