Isolation and structure elucidation of two new flavonoid glycosides from the infusion of Maytenus aquifolium leaves. Evaluation of the antiulcer activity of the infusion

Droplet countercurrent chromatography and high-performance liquid chromatog raphy fractionation of the aqueous infusion from Maytenus aquifolium Martiu s leaves afforded two flavonoid tetrasaccharides: quercetin 3-O-alpha-L-rha mnopyranosyl(1-->6)-O-[beta-D-glucopyranosyl(1-->3)-O-alpha-L-rhamnopranosy l( 1-->2)-O-beta-D-galactopyranoside and kaempferol 3-O-alpha-L-rhamnopyran osyl(1-->6)-O-[beta-D-glucopyranosyl( 1-->3)-O-alpha-L-rhamnopyranosy(1-->2 -2)-O-beta-D-galactopyranoside. All structures were elucidated by spectrosc opic methods. Pharmacological essays of the infusion showed antiulcer activ ity in rats.