Virgin olive oils from percolation (first extraction) have been compared wi
th the corresponding oils from centrifugation (second extraction). The form
er were characterized by (i) higher contents of total phenols, o-diphenols,
hydroxytyrosol, tyrosol-aglycons, tocopherols, trans-2-hexenal, total vola
tiles, and waxes; (ii) higher values of resistance to autoxidation and of t
urbidity; (iii) higher sensory scores; (iv) higher ratios of campesterol/st
igmasterol, traits-2-hexenal/hexanal, and trans-2-hexenal/total volatiles;
(v) lower contents of chlorophylls, pheophytins, sterols, and aliphatic and
triterpene alcohols; (vi) lower alcoholic index and color indices; (vii) s
imilar values of acidity, peroxide index, and UV (ultraviolet) spectrophoto
metric indices; (viii) similar percentages of saturated and unsaturated fat
ty acids, triglycerides, and diglycerides; and (ix) similar values of glyce
ridic indices. Stigmastadienes, trans-oleic, trans-linoleic, and trans-lino
lenic acid isomers were not detected in the two genuine oil kinds. Hence, t
he qualitative level of the first extraction oil was superior to the second
extraction one.