2,4-nonadienal and benzaldehyde bioantimutagens in Fushimi sweet pepper (Fushimi-Togarashi)

Fushimi sweet pepper, "Fushimi-togarashi'', is one of the "Kyo-yasai", trad itional vegetables, in Kyoto, Japan. The chloroform fraction of Fushimi swe et pepper showed bioantimutagenicity on UV induced mutation in Escherichia coli B/r WP2. The bioantimutagen was purified with silica gel chromatograph y and identified as 2,4-nonadienal (ID50 = 20 mu g/plate) on the basis of G C retention time and EI-MS spectrum of authentic 2,4-nonadienal. The sweet pepper also contained a known bioantimutagen, benzaldehyde (ID50 = 2 mg/pla te). Additive bioantimutagenicity was also observed by 2,4-nonadienal with benzaldehyde. 2,4-Nonadienal did not show bioantimutagenicity in an UV exci sion repair deficient strain, E. coli B/r WP2s uvrA(-). Furthermore no dela y of the first cell division after UV irradiation was observed in E. coli B /r WP2. These results indicate that the bioantimutagenic activity of 2,4-no nadienal. on UV mutagenesis might depend on the excision repair system in E . coli B/r WP2.