Fast separation of 16 seizure drug substances using non-aqueous capillary electrophoresis

A fast and simple method for separation of 16 seizure drug substances using capillary electrophoresis in a non-aqueous separation medium is described. The separation medium consists of a mixture of acetonitrile, methanol and glycerol with ammonium acetate/acetic acid as the electrolyte. The analytes are detected by UV detection at 214 nm. Injection from the detection end ( 8.5 cm to detector) combined with the usage of a short capillary (32.5 cm t otal length) makes it possible to separate all 16 amines within 2 min. The choice of solvents, electrolytes and viscosity increasing additives are dis cussed with special emphasis to their influence on the separation selectivi ty. (C) 1999 Elsevier Science B.V. All rights reserved.