Cm. Burns et al., Activation of rho dependent transcription termination by NusG - Dependenceon terminator location and acceleration of RNA release, J BIOL CHEM, 274(8), 1999, pp. 5245-5251
There is a kinetic limitation to Rho function at the first intragenic termi
nator in the lacZ gene (tiZ1) which can be overcome by NusG:Rho can termina
te transcription with slowly moving, but not rapidly moving, RNA polymerase
unless NusG is also present. Here we report further studies with two other
Rho dependent terminators that are not kinetically limited (tiZ2 and lambd
a tR1) which show that the requirement for NusG depends on the properties o
f the terminator and its location in the transcription unit. NusG is also s
hown to increase the rate of Rho-mediated dissociation of transcription com
plexes arrested at a specific termination stop point in the tiZ1 region and
the rates of dissociation with three different Rho factors and two differe
nt terminators correlated with their sensitivity to RNA polymerase elongati
on kinetics. These results suggest a model of NusG function which involves
an alteration in the susceptibility of the transcription complex to Rho act
ion which allows termination to occur within the short kinetic window when
RNA polymerase is traversing the termination region.