In vivo and in vitro association of 14-3-3 epsilon isoform with calmodulin: Implication for signal transduction and cell proliferation

Using a yeast two-hybrid screen, human 14-3-3 epsilon protein was found to interact with human calmodulin. In vitro binding assay between human 14-3-3 epsilon protein/peptide and calmodulin was demonstrated by native gel elec trophoresis, and the interaction was shown to be calcium dependent. Our res ults, along with the association of the 14-3-3 epsilon protein with other s ignaling proteins, suggest that the 14-3-3 protein could provide a link bet ween signal transduction and cell proliferation. J. Cell. Biochem. 73:31-35 , 1999. (C) 1999 Wiley-Liss, Inc.