Effect of dissolved oxygen tension and pH on the production of extracellular protease from a new isolate of Bacillus subtilis K2, for use in leather processing

Scale-up of production of an alkaline protease, previously characterised fr om a new isolate of Bacillus subtilis for use as a bating enzyme in leather processing, is described. Before large-scale commercial production of the protease is possible, characteristics of the growth of the bacterium and en zyme production in fermenters must be defined. In 2 dm(3) fermenters an opt imal specific activity of 296 x 10(3) U g(-1) cell dry weight was obtained after 60 h with the dissolved oxygen tension (DOT) kept above 10% and pH le ft uncontrolled. Culture pH was 6 on inoculation, falling to 5.3 after 12 h before rising steadily to similar to 8 at the end of fermentation. DOT was maintained above 10% by agitation in the range 300 to 500 rpm. The same cr iteria were adopted for scale-up to 20 dm(3) but the increase in activity o ccurred 24 h later. (C) 1999 Society of Chemical Industry.