Extruded oilseeds were fed to 24 dairy cows to study the influence on the c
onjugated linoleic acid content of milk and cheese. Cows were fed one of th
ree diets that contained forage and grain in a ratio of 47:53. A control di
et containing 13.5% soybean meal was compared with diets containing 12% ful
l fat extruded soybeans or 12% full fat extruded cottonseed. The control, e
xtruded soybean, and extruded cottonseed diets contained 2.73, 4.89, and 4.
56% fatty acids, respectively. Measurements were made during the last 5 wk
of the 8-wk experiment. The DM intakes and 3.5% fat-corrected milk yields w
ere higher for cows fed the extruded soybean and extruded cottonseed diets
than for cows fed the control diet. A tendency for lower fat and protein co
ntents in the milk of cows fed the extruded soybean and extruded cottonseed
diets was detected. Most of the Cls fatty acids were increased in the milk
and cheese when extruded soybeans and cottonseeds were fed. The conjugated
linoleic acid content in milk and cheese increased a mean of 109% when ful
l fat extruded soybeans were fed and increased 77% when cottonseeds were fe
d compared with the conjugated linoleic acid content when the control diet
was fed. Processing the milk into cheese did not alter the conjugated linol
eic acid content. The conjugated linoleic acid content of milk and cheese c
an be increased by the inclusion of full fat extruded soybeans and full fat
extruded cottonseeds in the diets of dairy cows.