Cloning of the sterol C-14 reductase gene of the tomato pathogenic fungus Septoria lycopersici and its complementation of the erg-3 mutation of Neurospora crassa

Authors
Aparna, K Sandrock, RW Kasbekar, DP
Citation
K. Aparna et al., Cloning of the sterol C-14 reductase gene of the tomato pathogenic fungus Septoria lycopersici and its complementation of the erg-3 mutation of Neurospora crassa, J GENETICS, 77(2-3), 1998, pp. 71-75
Citations number
26
Categorie Soggetti
Molecular Biology & Genetics
Journal title
JOURNAL OF GENETICS
ISSN journal
00221333 → ACNP
Volume
77
Issue
2-3
Year of publication
1998
Pages
71 - 75
Database
ISI
SICI code
0022-1333(199808/12)77:2-3<71:COTSCR>2.0.ZU;2-1
Abstract
We have cloned the erg-3 gene, which encodes the ergosterol biosynthetic en zyme sterol C-14 reductase, from the tomato pathogenic fungus Septoria lyco persici. Its nucleotide sequence, reported here, encodes a 512-amino-acid p olypeptide with 54% sequence identity to sterol C-14 reductase of Neurospor a crassa. The Septoria gene complemented the pisatin-sensitive, tomatine-re sistant and female-sterile phenotypes of a Neurospora erg-3 mutant.