Engagement of B cell receptor regulates the invariant chain-dependent MHC class II presentation pathway

The intracellular sites in which Ags delivered by the B cell receptor (BCR) are degraded and loaded onto class II molecules remain poorly defined. To address this issue, we generated wild-type and invariant chain (Ii)-deficie nt H-2(k) mice bearing BCR specific for hen egg lysozyme, Our results show that, 1) unlike Ags taken up from the fluid phase, Ii is required for prese ntation of hen egg lysozyme internalized through the BCR in a manner indepe ndent of the peptide analyzed; 2) BCR ligation induces intracellular accumu lation of MHC class II molecules only in Ii-positive B cells; and 3) these class II molecules reach intracellular compartments where BCR targets exoge nous Ag, No differences in expression of adhesion and costimulatory molecul es or in the presentation of soluble peptides were detectable between Ii-po sitive and -negative B cells. Therefore, the BCR delivers its ligand to com partments containing MHC class II-Ii complexes and bypasses the Ii-independ ent presentation pathway. The linked roles of Ag internalization and B cell activation of the BCR leads to potent Ii-dependent presentation in splenic B cells.