Separation of fluorescently derivatized deuterated isotopomers of phenylalanine using micellar electrokinetic chromatography and flow counterbalancedmicellar electrokinetic chromatography

Micellar electrokinetic chromatography (MEKC) and flow-counterbalanced MEKC separations of phenylalanine isotopomers are demonstrated. Using MEKC in a n untreated, bare, fused silica capillary, tetramethylrhodamine derivatized phenylalanine can be baseline separated from derivatized phenylalanines wi th five (aromatic ring) and eight (aromatic ring, alpha and beta carbon) de uteriums in 3.25 min. In addition, a mixture of phenylalanine isotopomers, synthesized through a partial deuteration of the amino acid's aromatic ring , was separated using flow counterbalanced MEKC (FC-MEKC). In these separat ions, phenylalanine isotopomers which differ by only one or two neutrons we re partially resolved. (C) 1999 John Wiley & Sons, Inc.