Identification of cyclopropene triacylglycerols by high-temperature gas chromatography and high-temperature gas chromatography-negative chemical ionization mass spectrometry

The seed oil of Choriosa speciosa, which is known to be rich in cyclopropen e triacylglycerols, has been investigated by high-temperature gas chromatog raphy using glass capillary columns coated with the stationary phase SOP-50 (50% diphenyl-50% dimethylpolysiloxane, methoxy terminated). Cyclopropene rings were characterized after a mild catalyzed hydrogenation procedure. Th e partial pi-character of the resulting cyclopropane ring supports the sepa ration of individual triacylglycerol species on the polarizable stationary phase. Characterization of the separated compounds was performed by negativ e chemical ionization-mass spectrometry with ammonia as reagent gas, (C) 19 99 John Wiley & Sons, Inc.