Molecular dynamics of the sodium channel pore vary with gating: Interactions between P-segment motions and inactivation

Disulfide trapping studies have revealed that the pore-lining (P) segments of voltage-dependent sodium channels undergo sizable motions on a subsecond time scale. Such motions of the pore may be necessary for selective ion tr anslocation. Although traditionally viewed as separable properties, gating and permeation are now known to interact extensively in various classes of channels. We have investigated the interaction of pore motions and voltage- dependent gating in mu 1 sodium channels engineered to contain two cysteine s within the P segments. Rates of catalyzed internal disulfide formation (k (ss)) were measured in K1237C+W1531C mutant channels expressed in oocytes. During repetitive voltage-clamp depolarizations, increasing the pulse durat ion had biphasic effects on the k(ss), which first increased to a maximum a t 200 msec and then decreased with longer depolarizations. This result sugg ested that occupancy of an intermediate inactivation state (I-M) facilitate s pore motions. Consistent with the known antagonism between alkali metals and a component of slow inactivation, k(ss) varied inversely with external [Na+](o). We examined the converse relationship, namely the effect of pore flexibility on gating, by measuring recovery from inactivation in Y401C + E 758C (YC/EC) channels. Under oxidative conditions, recovery from inactivati on was slower than in a reduced environment in which the spontaneous YC/EC cross-link is disrupted. The most prominent effects were slowing of a compo nent with intermediate recovery kinetics, with diminution of its relative a mplitude. We conclude that occupancy of an intermediate inactivation state facilitates motions of the P segments; conversely, flexibility of the P seg ments alters an intermediate component of inactivation.