Molecular biology of biotin attachment to proteins

Enzymatic attachment of biotin to proteins requires the interaction of a di stinct domain of the acceptor protein (the "biotin domain") with the enzyme , biotin protein ligase, that catalyzes this essential and rare post-transl ational modification. Both biotin domains and biotin protein ligases are ve ry strongly conserved throughout biology. This review concerns the protein structures and mechanisms involved in the covalent attachment of biotin to proteins.