F. De Pasquale et al., Minigrafting of shoots, roots, inverted roots, and somatic embryos for rescue of in vitro Citrus regenerants, J AM S HORT, 124(2), 1999, pp. 152-157
Citations number
24
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
Minigrafting was used for rescue of tissue culture regenerants of the follo
wing four species of Citrus: sour orange (C, aurantium L, 'AA CNR 31'), swe
et orange [C, sinensis (L,) Osb, 'Valencia Late'],lemon [C, lemon (L,) Burm
, 'Femminello Comune'] and mandarin (C, deliciosa Tenore 'Tardivo di Ciacul
li'), The grafting was carried out with different scion types including sho
ots, roots, inverted roots and somatic embryos, This material was obtained
in vitro from embryogenic style-derived callus, Seedlings of open-pollinate
d sour orange (C, aurantium L,), Cleopatra mandarin (C, reshni Hort, ex Tan
.) and 'Troyer' citrange [C, sinensis Osb. x Poncirus trifoliata (L,) Raf.]
were used as rootstocks, Minigrafting of shoots, roots, inverted roots and
embryos regenerated in vitro allowed successful rescue of these four speci
es. Percentages of successful minigrafts ranged from 100 % (shoots) to 2.5
% (inverted roots). The probability of successful graft unions Increased wi
th the age of the rootstock, The final mean canopy leaf area (120 days afte
r grafting) ranged from 5.2 cm(2) ('Tardivo di Ciaculli' mandarin grafted o
n 6 month old Cleopatra mandarin) to 157.9 cm(2) ('Valencia Late' sweet ora
nge grafted on 18-month-old Cleopatra mandarin). In this work we examined s
ome of the variables which influenced minigrafting and we determined the ef
ficacy of this method for rescue of in vitro regenerants of Citrus. This me
thod is also suggested as a technique to produce a high percentage of viabl
e plants from in vitro regenerants difficult to root.