PREVENTION OF MUCOSAL ESCHERICHIA-COLI INFECTION BY FIMH-ADHESIN-BASED SYSTEMIC VACCINATION

Virtually all uropathogenic strains of Escherichia coli, the primary c ause of cystitis, assemble adhesive surface organelles called type 1 p ili that contain the FimH adhesin. Sera from animals vaccinated with c andidate FimH vaccines inhibited uropathogenic E. coli from binding to human bladder cells in vitro. Immunization with FimH reduced in vivo colonization of the bladder mucosa by more than 99 percent in a murine cystitis model, and immunoglobulin G to FimH was detected in urinary samples from protected mice. Furthermore, passive systemic administrat ion of immune sera to FimH also resulted in reduced bladder colonizati on by uropathogenic E. coli. This approach may represent a means of pr eventing recurrent and acute infections of the urogenital mucosa.