Allelotyping of acute myelogenous leukemia: loss of heterozygosity at 7q31.1 (D7S486) and q33-34 (D7S498, D7S505)

Loss of a whole chromosome 7(-7) or a deletion of the long arm of chromosom e 7 del(7q) occurs frequently in many types of primary cancers including ca ses of acute myelogenous leukemia (AML). We analyzed for loss of heterozygo sity (LOH) of chromosome arm 7q in 26 AML cases using a set of 15 microsate llite markers in order to begin to determine the location of putative tumor suppressor genes (TSG) important to this disease. Seven samples (27%) show ed LOH at one or more loci on chromosome 7q. We identified the smallest com monly deleted regions to be at 7q31.1 (D7S486) and 7q33-34 (D7S498, D7S505) suggesting that alterations of a TSG in each region have an important role in de novo AML. (C) 1999 Elsevier Science Ltd. All rights reserved.