The cytoskeletal network controls c-Jun expression and glucocorticoid receptor transcriptional activity in an antagonistic and cell-type-specific manner
A. Oren et al., The cytoskeletal network controls c-Jun expression and glucocorticoid receptor transcriptional activity in an antagonistic and cell-type-specific manner, MOL CELL B, 19(3), 1999, pp. 1742-1750
The physical and functional link between adhesion molecules and the cytoske
letal network suggests that the cytoskeleton might mediate the transduction
of cell-to-cell contact signals, which often regulate growth and different
iation in an antagonistic manner. Depolymerization of the cytoskeleton in c
onfluent cell cultures is reportedly sufficient to initiate DNA synthesis,
Here we show that depolymerization of the cytoskeleton is also sufficient t
o repress differentiation-specific gene expression. Glutamine synthetase is
a glia-specific differentiation marker gene whose expression in the retina
l tissue is regulated by glucocorticoids and is ultimately dependent on gli
a-neuron cell contacts. Depolymerization of the actin or microtubule networ
k in cells of the intact retina mimics the effects of cell separation, repr
essing glutamine synthetase induction by a mechanism that involves inductio
n of c-Jun and inhibition of glucocorticoid receptor transcriptional activi
ty. Depolymerization of the cytoskeleton activates JNK and p38 mitogen-acti
vated protein kinase and induces c-Jun expression by a signaling pathway th
at depends on tyrosine kinase activity. Induction of c-Jun expression is re
stricted to Muller glial cells, the only cells in the tissue that express g
lutamine synthetase and maintain the ability to proliferate upon cell separ
ation. Our results suggest that the cytoskeletal network might play a part
in the transduction of cell contact signals to the nucleus.