The Cu2+-promoted cleavage of mRNA 5 '-cap analogs: A kinetic study with P-1-(7-methylguanosin-5 '-yl) P-3-(nucleosid-5 '-yl) triphospates and P-1-(7-methylguanosin-5 '-yl) P-4-(guanosin-5 '-yl) tetraphosphate

Authors
Wieczorek, Z Darzynkiewicz, E Kuusela, S Lonnberg, H
Citation
Z. Wieczorek et al., The Cu2+-promoted cleavage of mRNA 5 '-cap analogs: A kinetic study with P-1-(7-methylguanosin-5 '-yl) P-3-(nucleosid-5 '-yl) triphospates and P-1-(7-methylguanosin-5 '-yl) P-4-(guanosin-5 '-yl) tetraphosphate, NUCLEOS NUC, 18(1), 1999, pp. 11-21
Citations number
16
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
1
Year of publication
1999
Pages
11 - 21
Database
ISI
SICI code
0732-8311(1999)18:1<11:TCCOM5>2.0.ZU;2-H
Abstract
A kinetic study on the cleavage of a number of mRNA 5'-cap analogs, m(7)Gpp pN and m(7)GppppG, with Cu2+ aquo ion has been performed. Time-dependent pr oduct distributions at various pH and metal ion concentrations have been de termined by capillary zone electrophoresis, and these data have been used t o calculate the rate constants for various parallel reactions of the breakd own of the cap analogs.