Stability of phosphorothioate oligonucleotides in aqueous ammonia in presence of stainless steel

Authors
Turney, BJ Cheruvallath, ZS Andrade, M Cole, DL Ravikumar, VT
Citation
Bj. Turney et al., Stability of phosphorothioate oligonucleotides in aqueous ammonia in presence of stainless steel, NUCLEOS NUC, 18(1), 1999, pp. 89-93
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
1
Year of publication
1999
Pages
89 - 93
Database
ISI
SICI code
0732-8311(1999)18:1<89:SOPOIA>2.0.ZU;2-3
Abstract
Oligodeoxyribonucleotide phosphorothioates undergo desulfurization in prese nce of metal in aqueous ammonia at elevated temperatures. The extent of sul fur loss and chain cleavage have been investigated.