The fluorescence characteristics of the Schiff bases 2-(3- pyridylmethylimi
nomethyl)phenol (1), 2-(2-pyridyliminomethyl)phenol (2), N,N'-bis(salicylid
ene)-2,3-pyridinediamine (3), N,N'-bis(salicylidene)-2:6-pyridinediamine (4
) and 2-(2-amino-4-methoxymethyl-6-methyl-3 -pyridylmethyliminomethyl)pheno
l (5) were studied in various solvents at different pH values. Correspondin
g quantum efficiencies were determined. Compound 1, which showed a tendency
towards tautomeric interconversion to ketoamine in polar protic solvents w
as not fluorescent at pH < 8. The fluorescence of other compounds was very
sensitive to solvent polarity and the pH of the medium. Compounds 2 - 4, pr
eferably present as enolimines in all solvents, were not fluorescent in non
-polar and moderately polar solvents, whereas weak emission was observed in
polar solvents, like methanol, dimethylformamide and dioxane/water 1/1 (0.
001 < Q < 0.072). A significant increase in Stokes shifts and in quantum ef
ficiencies was noted as a result of increasing polarity of dioxane/water mi
xtures, indicating specific interactions with polar water molecules The emi
ssion Has promoted at acidic pH values where a pyridinium cation was found
(0.061 < Q < 0.519. in dioxane/water 1/1 at pH 3.4) Compound 5. which was a
tautomeric mixture of enolimine and cyclic diamine in all solvents. was fl
uorescent in polar as well as in non-polar media The quantum efficiency) va
ried dependent on the: solvent and pH (0.023 < Q) < 0.435). The cyclic diam
ine. i. e, the more rigid structure was supposed to be responsible for the
fluorescence in non-polar and aprotic solvents as well as at neutral and we
akly basic pH values.