Pd. Nagy et al., Mapping sequences active in homologous RNA recombination in brome mosaic virus: Prediction of recombination hot spots, VIROLOGY, 254(1), 1999, pp. 92-104
The mechanism of homologous recombination has been studied previously in br
ome mosaic virus (BMV), a tricomponent, positive-stranded RNA virus of plan
ts, by using artificial sequences (reviewed by J. J. Bujarski and P. D. Nag
y (1996). Semin. Virol. 7, 363-372). Here we extend these studies over BMV-
derived sequences to obtain clues on prediction of homologous recombination
hot spots. First, mismatch mutations, which reduced the AU content, were i
ntroduced into the common 60-nt recombination hot-spot sequence, either in
the RNA2 or in both RNA2 and RNAS components. This decreased the frequency
of targeted homologous RNA2/RNA3 recombination and changed the distribution
of junction sites. Second, several short BMV RNA1- or RNA2-derived sequenc
es were introduced into the RNA3 component, and homologous recombination ac
tivity of these sequences was compared with that observed for previously ch
aracterized artificial sequences. Third, sequences at homologous recombinan
t junctions were compared among a large number of targeted and nontargeted
recombinants. All these studies revealed several factors important for homo
logous recombination including the length of sequence identity, the extent
of sequence identity, the AU content of the common sequences, the relative
position of the AU-rich segment vs a GC-rich segment, and the presence of G
C-rich sequences. Based on this novel model, we suggest that recombination
hot spots can be predicted by means of RNA sequence analysis. In addition,
we show that recombination can occur between positive and negative strands
of BMV RNAs. This provides further clues toward the mechanism of recombinat
ion processes in BMV. (C) 1999 Academic Press.