Pulsed field gel electrophoresis of restriction endonuclease digested genom
ic DNA from a collection of clinical isolates of Rhodococcus equi was used
to compare strain diversity on different Thoroughbred horse farms over time
. Restricted diversity was found among the isolates tested, as the same str
ains were detected on multiple farms and in multiple years. Marked variatio
n occurred in strain prevalence with some strains being represented by sing
le isolates, and the most prevalent by 26 isolates. There were dominant str
ains on some farms and the prevalence of some strains differed between farm
s. Infection with multiple strains was noted in some cases where multiple i
solates from a single foal were examined.