Isolation of a frog lectin using polylysine to reduce non-specific interactions with heparin binding ligand

Polylysine is a polycation used in histology to glue tissue sections to gla ss slides. It was successfully used to eliminate positively charged protein s from heparin-agarose beds applied for affinity chromatography facilitatin g the preparative steps in the isolation and purification of heparin bindin g lectins from the ovary of the frog Leptodactylus occellatus. The addition pretreatment of agarose-heparin beds with polylysine increased more than 3 -fold the hemagglutinating capacity of eluted lectin.