Identification of a human cDNA encoding a novel protein structurally related to the yeast membrane-associated metalloprotease, Ste24p

Recently, a novel membrane-associated metalloprotease, designated Ste24p, h as been identified in Saccharomyces cerevisiae [K. Fujimura-Kamada, F.J. No uvet, S. Michaelis, J. Cell Biol. 27 (1997) 271-285]. We cloned a human bra in cDNA encoding a protein homologous to Ste24p (designated Hs Ste24p). The predicted 475-amino acid product of its open reading frame exhibited 62% s imilarity to Ste24p, and contained a zinc metalloprotease motif (HEXXH) and multiple predicted membrane spans. Northern blot analysis showed that this gene was expressed in most tissues. Immunofluorescence analysis of epitope -tagged Hs Ste24p constructs suggested that it is localized in the ER and p ossibly also in the Golgi compartment; A search of the expression sequence tag database identified a fragment of DNA encoding a segment homologous to the segment of Hs Ste24p containing the HEXXH motif in insects and nematode s. Thus, Ha Ste24p could be a member of a new family of Ste24p-like membran e-associated metalloproteases which are widely conserved in eukaryotes. (C) 1999 Elsevier Science B.V. All rights reserved.