Citation
V. Stoppin-mellet et al., Tobacco BY-2 cell-free extracts induce the recovery of microtubule nucleating activity of inactivated mammalian centrosomes, BBA-MOL CEL, 1449(1), 1999, pp. 101-106
Citations number
25
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
ISSN journal
01674889
→ ACNP
Volume
1449
Issue
1
Year of publication
1999
Pages
101 - 106
Database
ISI
SICI code
0167-4889(19990204)1449:1<101:TBCEIT>2.0.ZU;2-I
Abstract
The structure and the molecular composition of the microtubule-organizing c
enters in acentriolar higher plant cells remain unknown. We developed an in
vitro complementation assay where tobacco BY-2 extracts can restore the mi
crotubule-nucleating activity of urea-inactivated mammalian centrosomes. Ou
r results provide first evidence that soluble microtubule-nucleating factor
s are present in the plant cytosolic fraction. The implication for microtub
ule nucleation in higher plants is discussed. (C) 1999 Elsevier Science B.V
. All rights reserved.