Western blotting for ginseng saponins, ginsenosides using anti-ginsenosideRb1 monoclonal antibody

Authors
Fukuda, N Tanaka, H Shoyama, Y
Citation
N. Fukuda et al., Western blotting for ginseng saponins, ginsenosides using anti-ginsenosideRb1 monoclonal antibody, BIOL PHAR B, 22(2), 1999, pp. 219-220
Citations number
19
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
09186158 → ACNP
Volume
22
Issue
2
Year of publication
1999
Pages
219 - 220
Database
ISI
SICI code
0918-6158(199902)22:2<219:WBFGSG>2.0.ZU;2-6
Abstract
Ginsenosides separated by silica gel TLC were blotted to a polyvinylidene d ifluoride (PVDF) membrane which was treated with a NaIO4 solution followed by bovine serum albumine (BSA), resulting in a ginsenoside-BSA conjugate on a PVDF membrane. The blotted bands were stained with monoclonal antibody ( MAb). The newly established Western blotting method was used for the determ ination of ginsenosides and their distribution in various Panax species.