Western blotting method for the immunostaining detection of glucuronides of glycyrrhetic acid using anti-glycyrrhizin monoclonal antibody

A method for detecting glucuronides of glycyrrhetic acid using Western blot ting was investigated. Glucuronides of glycyrrhetic acid separated by silic a gel TLC were transferred to a polyvinylidene difluoride membrane. The mem brane was treated with sodium periodate solution followed by bovine serum a lbumin, resulting in a glucuronides of glycyrrhetic acid-BSA conjugate. Ind ividual spots were stained by monoclonal antibody against glycyrrhizin. Imm unostaining of glucuronides of glycyrrhetic acid was more sensitive compare d to other staining methods. The newly established immunostaining method ca n be expanded to the distribution of glucuronides of glycyrrhetic acid in t he plant body.