Retinoic acid stimulates the expression of 11 beta-hydroxysteroid dehydrogenase type 2 in human choriocarcinoma JEG-3 cells

The syncytiotrophoblasts of the human placenta express high levels of 11 be ta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2), the enzyme responsib le for the inactivation of glucocorticoids. It has been proposed that the p lacental 11 beta-HSD2 serves as a barrier to protect the fetus from high le vels of maternal cortisol. To examine the hypothesis that nutritional signa ls regulate the expression of 11 beta-HSD2 in placental syncytiotrophoblast s, we investigated the effects of retinoic acids (RAs), the major metabolit es of vitamin A, on the expression of 11 beta-HSD2 using human choriocarcin oma JEG-3 cells as a model. This trophoblast-like cell line displays a numb er of functional similarities to the syncytiotrophoblast. Treatment for 24 h with all-trans RA (1-1000 nM) resulted in a dose-dependent increase in 11 beta-HSD2 activity with a maximal effect (increase to 3-fold) at 100 nM. T he effect of all-trans RA (100 nM) was also time-dependent in that the effe ct was detectable at 6 h and reached its maximum by 48 h. Similar increases in 11 beta-HSD2 activity were observed when the cells were treated with 9- cis RA. Results from semiquantitative reverse transcription-polymerase chai n reaction demonstrated that there was a corresponding increase in 11 beta- HSD2 mRNA after RA treatment. Moreover, treatment with actinomycin D (100 n g/ml) abrogated the increase in 11 beta-HSD2 mRNA induced by RA, indicating an effect on transcription. In conclusion, the present study has demonstra ted for the first time that RA, at physiological concentrations, induces 11 beta-HSD2 gene expression and enzyme activity in JEG-3 cells. If this occu rs in vivo, the present finding suggests that high expression of 11 beta-HS D2 in the human placenta may be maintained, at least in part, by dietary in take of vitamin A.