Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin

Authors
Ichinose, K Surti, C Taguchi, T Malpartida, F Booker-Milburn, KI Stephenson, GR Ebizuka, Y Hopwood, DA
Citation
K. Ichinose et al., Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin, BIOORG MED, 9(3), 1999, pp. 395-400
Citations number
17
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
9
Issue
3
Year of publication
1999
Pages
395 - 400
Database
ISI
SICI code
0960-894X(19990208)9:3<395:PTTAGR>2.0.ZU;2-A
Abstract
Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces co elicolor A3(2) was studied using the act cluster deficient strain, CH999, c arrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate, (S)-DNPA, suggesting that the actVI-ORF1 product is a reductase determinin g the C-3 stereochemical centre. (C) 1999 Elsevier Science Ltd. All rights reserved.