Inhibition of human cytomegalovirus protease by enedione derivatives of thieno[2,3-d]oxazinones through a novel dual acylation/alkylation mechanism

Enedione derivatives of thieno[2,3-d]oxazinones are nanomolar inhibitors of CMV protease which act through a novel dual acylation of the catalytic ser ine and alkylation of the protease cysteine 161 via a Michael addition to t he enedione moiety of the inhibitor. (C) 1999 Elsevier Science Ltd. All rig hts reserved.