Effect of ED-71 on modeling of bone in distraction osteogenesis

Citation
K. Yamane et al., Effect of ED-71 on modeling of bone in distraction osteogenesis, BONE, 24(3), 1999, pp. 187-193
Citations number
16
Categorie Soggetti
Endocrynology, Metabolism & Nutrition","da verificare
Journal title
BONE
ISSN journal
87563282 → ACNP
Volume
24
Issue
3
Year of publication
1999
Pages
187 - 193
Database
ISI
SICI code
8756-3282(199903)24:3<187:EOEOMO>2.0.ZU;2-#
Abstract
We investigated the effect of 2-beta-(3-hydroxypropoxy)-1 alpha,25-dihydrox yvitamin D-3 (ED-71) on the modeling of bone in distraction osteogenesis, T he tibiae of 30 rabbits were lengthened by 10 mm in 10 days. Following oste otomy, ED-71 (0.05 mu g/kg) was administered subcutaneously twice a week to the ED-71 group until necropsy, The bone mineral content (BMC) of the leng thened callus was measured by dual-energy X-ray absorptiometry (DXA), Five rabbits per group were killed at 1, 3, and 8 weeks after completion of leng thening, and the lengthened callus was examined histologically and histomor phometrically. Bone volume of the lengthened callus was measured by periphe ral quantitative computed tomography (pQCT) at 8 weeks after the completion of lengthening. At all timepoints the BMC in the ED-71 group was significa ntly higher than that in the untreated group. The mineral apposition rate a nd bone formation rate were higher in the ED-71 group than in the untreated group at 1 and 3 weeks after the completion of lengthening on the coronal section, In cross sections, the cortical area and width in the ED-71 group showed significantly higher values than in the untreated group at 8 weeks a fter the completion of lengthening, Both the endosteal osteoid surface and endosteal eroded surface showed no differences between groups. However, the endosteal mineral apposition rate and endosteal bone formation rate were s ignificantly higher in the ED-71 group. At 8 weeks after completion of leng thening, the intracortical area and intracortical BMC were significantly gr eater in the ED-71 group than in the untreated group, but no significant di fference was noted in intracortical BMD. These findings indicate that ED-71 increases callus volume during the early period after the completion of le ngthening, resulting in thick cortical bone formation. (Bone 24:187-193; 19 99) (C) 1999 by Elsevier Science Inc. All rights reserved.