Fas antigen is a cell-surface protein that transduces an apoptotic signal f
rom the cell surface into the cytoplasm, The localization of Fas antigen in
human oral squamous cell carcinoma (SCC) was examined by immunohistochemis
try using a monospecific polyclonal antibody with a high titre, This antibo
dy, designated as Fas D, was raised against a synthetic polypeptide segment
corresponding to a specific extracellular domain of human Fas antigen (aa
104-114).
Thirty-eight specimens of oral SCCs were stained with Fas D antibody and 26
(68%) reacted intensely. The specimens were graded as 'well', 'moderately'
, or 'poorly differentiated', according to the histopathological criteria.
Out of 24 cases of the well differentiated tumours examined, 22 had reacted
to Fas staining. The tumour cells formed nests that encompassed keratin pe
arls; staining was confined to cytoplasmic granules of peripheral cells. Am
ong the moderately differentiated tumours, 4 out of 11 cases had reacted to
Fas staining. No Fas-positive cells were observed in the poorly differenti
ated tumours, but only three specimens were examined. The expression of Fas
antigen seems to be related to the degree of tumour differentiation.