Separation of hydrophobic compounds by nonaqueous micellar electrokinetic chromatography using formamide solvent

In this study, some uncharged hydrophobic compounds, such as p-arylacetophe nones, polycyclic aromatic hydrocarbons and steroids, were separated by mic ellar electrokinetic chromatography (MEKC) using amide solvents containing sodium n-dodecyl sulfide (SDS), di-(2-ethylhexyl) sodium sulfosuccinate (AO T) or taurodexycholic acid sodium salt (STDC). The separation was shown to be dependent on the types of amide solvent and the surfactant concentration . Three different amides, formamide (FA), N-methyl-formamide (NMF) and N,N- dimethylformamide (DMF) were used as solvent. A satisfactory result was obt ained in formamide/SDS/NaH2PO4 medium. The effects of the amide solvent, su rfactant and electrolyte on the separation were also studied.