PARTIAL-PURIFICATION AND CHARACTERIZATION OF SUCROSE-PHOSPHATE SYNTHASE FROM PRECLIMACTERIC AND CLIMACTERIC BANANAS

Authors
NASCIMENTO JRO CORDENUNSI BR LAJOLO FM
Citation
Jro. Nascimento et al., PARTIAL-PURIFICATION AND CHARACTERIZATION OF SUCROSE-PHOSPHATE SYNTHASE FROM PRECLIMACTERIC AND CLIMACTERIC BANANAS, Journal of agricultural and food chemistry, 45(4), 1997, pp. 1103-1107
Citations number
31
Categorie Soggetti
Food Science & Tenology",Agriculture,"Chemistry Applied
Journal title
Journal of agricultural and food chemistry → ACNP
ISSN journal
00218561
Volume
45
Issue
4
Year of publication
1997
Pages
1103 - 1107
Database
ISI
SICI code
0021-8561(1997)45:4<1103:PACOSS>2.0.ZU;2-M
Abstract
Sucrose phosphate synthase (SPS) (EC 2.4.1.14) was purified from banan as (Musa acuminata cv. Nanicao) at different developmental and ripenin g stages (70, 110, and 130 days after anthesis), corresponding to tiss ue with different structure and composition. Banana SPS exists in thre e polymeric forms as observed by electrophoresis and immunoblotting, s imilar in all stages studied, with M-W respectively of 180, 254, and 6 86 kDa. The three forms have the same 116 kDa subunit, and their activ ity increased during ripening associated with increased protein synthe sis as shown by Western blotting.