D. Makeiff et al., DETERMINATION OF ZYGACINE IN ZIGADENUS-VENENOSUS (DEATH CAMAS) BY IMAGE-ANALYSIS ON THIN-LAYER CHROMATOGRAPHY, Journal of agricultural and food chemistry, 45(4), 1997, pp. 1209-1211
A new method, utilizing image analysis on TLC, was developed for the d
etermination of zygacine, the neurotoxic steroidal alkaloid in death c
amas (Zigadenus venenosus). The quantitative detection level of zygaci
ne on TLC was 1 mu g. Diurnal changes in zygacine levels were not evid
ent, nor were there differences in zygacine levels in death camas grow
ing at high- or low-elevation habitats. The image analysis method was
also applied to the determination of vanillylveracevine in Zigadenus e
legans.