Quantification of trovafloxacin in serum by high-performance liquid chromatography with on-line solid-phase extraction

A rapid. simple, accurate and sensitive liquid chromatographic assay with o n-line solid-phase extraction is described for determination of trovafloxac in in human serum. Samples were deproteinized with acetonitrile and injecte d on to an NH2 extraction column for sample clean-up. Thereafter, an on-lin e column-switching sys tem was used for quantitative transfer of the drug t o a Cls analytical column. Separation was performed by ion-pair chromatogra phy and detection was by ultraviolet absorbance at 275 nm. Recovery was 98. 5 %. The linear range was from 0.25 to 20 mu g mL(-1), with a correlation c oefficient of 0.999. Detection limit was 0.1 mu g mL(-1) from extraction of 25 mu L serum.