Genetic relationship among 19 accessions of six species of Chenopodium L.,by Random Amplified Polymorphic DNA fragments (RAPD)

Citation
Pm. Ruas et al., Genetic relationship among 19 accessions of six species of Chenopodium L.,by Random Amplified Polymorphic DNA fragments (RAPD), EUPHYTICA, 105(1), 1999, pp. 25-32
Citations number
31
Categorie Soggetti
Plant Sciences
Journal title
EUPHYTICA
ISSN journal
00142336 → ACNP
Volume
105
Issue
1
Year of publication
1999
Pages
25 - 32
Database
ISI
SICI code
0014-2336(1999)105:1<25:GRA1AO>2.0.ZU;2-W
Abstract
The RAPD technique was used to identify genetic relationships in 19 accessi ons, including six species of the genus Chenopodium. A dendrogram was const ructed using UPGMA from 399 DNA markers. The molecular data clustered speci es and accessions into five different groups. Group 1 with three cultivated varieties of C. nuttalliae, Group 2 included eight cultivars and two wild varieties of C. quinoa, Group 3 with C. berlandieri and C. album, Group 4 w ith two accessions of C, pallidicaule, and Group 5 with 2 accessions of C. ambrosioides. The polymorphic patterns generated by RAPD profiles showed di fferent degrees of genetic relationship among the species studied. A low le vel of intraspecific variation was found within the accessions of C. quinoa , C. nuttalliae, and C. pallidicaule. The RAPD markers were found to be a u seful tool for detecting genetic variation within the genus Chenopodium.