Pm. Ruas et al., Genetic relationship among 19 accessions of six species of Chenopodium L.,by Random Amplified Polymorphic DNA fragments (RAPD), EUPHYTICA, 105(1), 1999, pp. 25-32
The RAPD technique was used to identify genetic relationships in 19 accessi
ons, including six species of the genus Chenopodium. A dendrogram was const
ructed using UPGMA from 399 DNA markers. The molecular data clustered speci
es and accessions into five different groups. Group 1 with three cultivated
varieties of C. nuttalliae, Group 2 included eight cultivars and two wild
varieties of C. quinoa, Group 3 with C. berlandieri and C. album, Group 4 w
ith two accessions of C, pallidicaule, and Group 5 with 2 accessions of C.
ambrosioides. The polymorphic patterns generated by RAPD profiles showed di
fferent degrees of genetic relationship among the species studied. A low le
vel of intraspecific variation was found within the accessions of C. quinoa
, C. nuttalliae, and C. pallidicaule. The RAPD markers were found to be a u
seful tool for detecting genetic variation within the genus Chenopodium.