The 3 ' terminal sequence of RNA1 of wheat spindle streak mosaic virus Canadian isolate (WSSMV-C)

The sequence of the 3' terminal 1722 nucleotides (nts) of RNA1 of the type (Canadian) isolate of wheat spindle streak mosaic bymovirus (WSSMV-C) was d etermined. The sequence started within a single open reading frame (ORF), w hich was expected to encode the carboxyl terminus of the nuclear inclusion b protein (NIb) and the capsid protein (CP) of 294 amino acids, followed by a 3' untranslated region (UTR) of 237 nucleotides. The NIb and CP of WSSMV -C share 99 and 100% amino acid sequence identity with the corresponding pr oteins of WSSMV-French isolate (WSSMV-F), but only 89 and 77% with wheat ye llow mosaic virus (WYMV-J), respectively. The 3'UTR of RNA1 of WSSMV-C shar es 94% nucleotide sequence identity with that of WSSMV-F but only 73% with WYMV-J and WYMV-Chinese isolate (WYMV-Chi). The results support the classif ication of WSSMV-C and WSSMV-F as strains of the same virus species which i s distinct from WYMV.