Extractive nitrogenous constituents of olive flounder Paralichthys olivaceus cultured by recombinant bovine somatotropin

In order to investigate the composition of extractive nitrogenous constitue nts in the olive flounder Paralichthys olivaceus cultured by recombinant bo vine somatotropin (BST) supplemented-feed and the BST unsupplemented-feed ( control) at the south coast of Korea, the muscle was analyzed for extractiv e nitrogen, free amino acids, oligopeptides, nucleotides and related compou nds, quaternary ammonium bases, and guanidino compounds using specimens col lected from April 1996 to March 1997. Free amino acids such as taurine, ala nine, serine, threonine, lysine, cystathionine, and glutamic acid were rich in all the samples. Among them taurine was the most abundant, occupying fr om 9 to 38%. As for nucleotides and related compounds, inosine 5'-monophosp hate was the principal constituent in all the samples. beta-Alaninebetaine, homarine were detected in all the samples but glycinebetaine was found onl y in some samples. The large amount of creatine was detected in all samples from 305 to 545 mg/100 g muscle. There was no conspicuous difference in th e extractive nitrogenous constituents between the two group of samples when compared with the amount of free amino acids, nucleotides and related comp ounds, bataines, trimethylamine oxide (TMAO) and trimethylamine (TMA), and creatine and creatinine, except the results of oligopeptides and sensory ev aluation (p<0.10).